CMV (Cytomegalovirus) Nucleic Acid detection (PCR)


Test Name
CMV (Cytomegalovirus) Nucleic Acid detection (PCR)
Test Code
CMPC1
Specimen Type

DEDICATED molecular specimens are required for blood and swab requests

Amniotic fluid, Breastmilk, CSF, EDTA blood, Plasma/serum, Faeces, NST Card (Guthrie Card), Dry swab, Saliva/Saliva swab, Semen, Tissue biopsy, Urine

Minimum Volume
0.5 mL bloods / 0.2 mL CSF
Preferred Volume
1.0 mL bloods / 0.5 mL CSF / Saliva - dry swab soaked with Saliva
Comments

Quantitative PCR (viral loads) are performed on all detected blood specimens. Note: These are performed on plasma unless whole blood is specified or is insufficient to be performed on plasma.

Saliva Collection: Do not collect sample within 1 hour of breast feeding. 

Laboratory Staff:
Guthrie Card test:
Register with lab number and scan all paperwork. Send to VCGS for retrieval of NST card (Guthrie card). Phone log time of retrieval request dispatch and time of Guthrie card receipt before forwarding to Molecular Microbiology lab for testing.
NOTE: Paperwork must include:
1. Dr request 
2. Parent consent for VCGS to process request

Recommended transport/storage conditions

  • Amniotic fluid: 2-8oC for 3 days
  • Breast milk: 2-8oC for 3 days
  • CSF: 2-8oC for 3 days
  • EDTA Blood: 2-8oC for 3 days
  • Faeces: 2-8oC for 3 days
  • NST Card (Guthrie Card): Less than <30oC (Room temp)
  • Plasma/Serum: 2-8oC for 3 days
  • Saliva/ Saliva swab: <30oC (Room temp) for 3 days
  • Semen: 2-8oC for 3 days
  • Dry swab: <30oC (Room temp) for 3 days
  • Tissue biopsy: 2-8oC for 3 days
  • Urine: Transport at RT for 24 hours, 2-8oC for 4 days
Assay Performed
Molecular Microbiology
RCH
Level 4, 50 Flemington Rd, Parkville 3052
Laboratory Hours: Mon-Fri 8:30am - 17:30 

No after hours service; if urgent testing is required, the requesting clinician is to contact on-call Clinical Microbiologist for advice via switchboard
9345 4673
Assay Frequency

Daily (Monday - Friday)

For same day results, specimens need to be received in the laboratory before 11:00am (RCH) AND 8:30am (RWH)

ABO Titre
EDTA 2.7


CMV Nucleic Acid detection